What Are the Cutoff Levels for a DOT Urine Drug Test?

DOT urine drug test cutoff levels are set in a table in 49 CFR 40.85(a), with one cutoff for the initial test and one for the confirmatory test. All values are in nanograms per milliliter (ng/mL). A result below the cutoff is reported as negative.

• Marijuana metabolites (THCA): 50 initial, 15 confirmatory
• Cocaine metabolite (benzoylecgonine): 150 initial, 100 confirmatory
• Codeine and morphine: 2000 initial, 2000 confirmatory
• Hydrocodone and hydromorphone: 300 initial, 100 confirmatory
• Oxycodone and oxymorphone: 100 initial, 100 confirmatory
• 6-Acetylmorphine: 10 initial, 10 confirmatory
• Phencyclidine (PCP): 25 initial, 25 confirmatory
• Amphetamine and methamphetamine: 500 initial, 250 confirmatory
• MDMA and MDA: 500 initial, 250 confirmatory

Footnotes to the table cover grouped analytes, meaning two or more drugs in the same class with the same initial cutoff. When a laboratory uses an alternate technology initial test that is specific for THCA or benzoylecgonine, it uses the confirmatory cutoff for both tests, 15 ng/mL for THCA and 100 ng/mL for benzoylecgonine.

Under 40.85(d), the laboratory reports quantitative values for morphine or codeine at 15,000 ng/mL or above, and under 49 CFR 40.97(g) it also provides quantitative values for confirmed positive morphine or codeine urine results at or below 15,000 ng/mL. The drugs in the DOT panel are listed in What Drugs Are Included in a DOT Drug Test?. Oral fluid specimens have their own, lower cutoffs, covered in What Are the Cutoff Levels for a DOT Oral Fluid Drug Test?.

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