A DOT drug test covers five drugs or classes of drugs, according to 49 CFR 40.82. The listed drugs are marijuana metabolites, cocaine metabolites, amphetamines, opioids and phencyclidine (PCP).
The same section states that laboratories must not test DOT specimens for any other drugs. The cutoff concentrations that laboratories use for initial and confirmatory urine tests are set out in a table in 49 CFR 40.85.
Under 49 CFR 40.13, a DOT specimen may not be tested for additional drugs. Any non-DOT test must be kept completely separate from the DOT test and use a separate specimen.
The DOT specimen is collected at a collection site and tested by a laboratory. Both provider types can be found in the MyDrugTesting.com directory by searching by location and service.
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