Appendix A to 49 CFR Part 40 sets the specifications. The collection container holds at least 55 mL of urine. Each specimen bottle holds at least 35 mL, or, when the bottles are two distinct sizes, the primary bottle holds at least 35 mL and the split bottle at least 20 mL.
Under Appendix A, the collection container:
- Is a single-use plastic container large enough to easily catch and hold at least 55 mL of urine voided from the body
- Has graduated volume markings clearly noting levels of 45 mL and above
- Has a temperature strip with graduated readings of 32 to 38 °C (90 to 100 °F), affixed or able to be affixed at a proper level on the outside, or another method such as a temperature device built into the container wall, provided the measurement is accurate and there is no potential for contamination of the specimen
- Is individually wrapped in a sealed plastic bag or shrink wrapping, or has a peelable sealed lid or other easily visible tamper-evident system
Under Appendix A, the plastic specimen bottles:
- Have screw-on or snap-on caps that prevent seepage of urine from the bottles during shipment
- Have markings clearly showing the 30 mL level for the primary specimen and the 15 mL level for the split
- Are designed so that the tamper-evident bottle seals on the CCF fit with no damage to the seal when the employee initials it and no chance that the seal overlap conceals printed information
- Are wrapped with caps, together or individually, in sealed plastic bags or shrink wrapping separate from the collection container, or have a peelable sealed lid or other easily visible tamper-evident system
- Are made of leach-resistant plastic
How the temperature strip is read is covered in How Is a Urine Specimen's Temperature Checked at a DOT Collection?, and how the collector pours and seals the bottles is covered in How Is a DOT Urine Specimen Split and Sealed?. The full kit list is in What Must a DOT Urine Collection Kit Include?.
Learn Laws® offers DOT Urine Specimen Collector Error Correction Training, an online error correction course for DOT urine collectors after a cancelled test, with three live mock collections under 49 CFR 40.33(f) within 30 days.
MyDrugTesting.com supplier profiles carry category labels, such as Labs or Trainers, at the head of the page.