What Must a DOT Urine Collection Kit Include?

A DOT urine collection kit includes a collection container, plastic specimen bottles, a leak-resistant plastic bag, absorbent material and a shipping container. Under 49 CFR 40.44, each DOT drug test uses a collection kit that meets the requirements of Appendix A to Part 40, titled DOT Standards for Urine Collection Kits.

Appendix A describes the five components:

• Collection container, a single-use plastic container large enough to easily catch and hold at least 55 mL of urine
• Plastic specimen bottles for the primary and split specimens
• Leak-resistant plastic bag with two sealable compartments or pouches, one large enough to hold two specimen bottles and the other large enough to hold the CCF paperwork
• Absorbent material, enough to absorb the entire contents of both specimen bottles and designed to fit inside the bag pouch that holds the bottles
• Shipping container that adequately protects the specimen bottles from shipment damage, such as a standard courier box, small cardboard box or plastic container

Under Appendix A, the bag is sealed in a way that makes any tampering or attempt to open either compartment evident. The collection container may be made available separately at collection sites to address shy bladder situations when several voids may be required, and the shipping container may also be made available separately.

The container and bottle specifications are covered in What Are the DOT Specifications for Urine Collection Containers and Specimen Bottles?, and shipping is covered in When Is a Shipping Container Required for DOT Drug Test Specimens?. The kit is used for specimens sent to a laboratory, because 49 CFR 40.210 does not authorize point-of-collection or instant tests for DOT drug testing, as covered in What Types of Drug Tests Are Allowed under DOT Rules?.

Learn Laws® offers DOT Urine Specimen Collector Training, an online course with qualification training and five live mock collections under 49 CFR 40.33.

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